SPECTROPHOTOMETRY ASSAY OF LIPASE ACTIVITY USING RHODAMINE-6G

被引：0

作者：

VANAUTRYVE, P

RATOMAHENINA, R

RIAUBLANC, A

MITRANI, C

PINA, M

GRAILLE, J

GALZY, P

机构：

来源：

OLEAGINEUX | 1991年 / 46卷 / 01期

关键词：

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A method for lipase activity determination by spectrophotometry is described. Rhodamine 6G reagent is used for complexation with the free fatty acids liberated during lipolysis. This method uses toluene as the Rhodamine 6G solvent. In the presence of fatty acids, a pink coloration appears and absorbance is read at 513 nm. It is shown this method is pH independent and gives results similar to those obtained with TCL technique. This method is utilized for lipase activity determination in a yeast strain Candida steatolytica.

引用

页码：29 / 31

页数：3