beta-Amyloid selectively augments NMDA receptor-mediated synaptic transmission in rat hippocampus

被引：109
作者：
Wu, JQ
Anwyl, R
Rowan, MJ
机构：
[1] TRINITY COLL DUBLIN,DEPT PHARMACOL & THERAPEUT,DUBLIN 2,IRELAND
[2] TRINITY COLL DUBLIN,DEPT PHYSIOL,DUBLIN 2,IRELAND
来源：
NEUROREPORT | 1995年 / 6卷 / 17期
基金：
英国惠康基金;
关键词：
beta-amyloid; glutamate; N-methyl-D-aspartate (NMDA) receptor; excitatory synaptic transmission; hippocampus; dentate granule cell; Alzheimer's disease;
D O I：
10.1097/00001756-199511270-00031
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
THE effect of beta-amyloid on alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor-mediated glutamatergic excitatory postsynaptic currents measured with whole-cell voltage-clamp techniques, was investigated in the rat dentate gyrus in vitro. Application of beta-amyloid (1-40) by extracellular perfusion (200nM) or intracellularly via the recording pipette (100nM) resulted in a gradual enhancement of the NMDA receptor-mediated synaptic currents which did not reverse on washout. Basal AMPA receptor-mediated transmission, resting membrane potential or input resistance of the granule cells did not change. These results provide direct evidence that beta-amyloid selectively interacts with the potentially neurotoxic NMDA receptor via a postsynaptic site. It is proposed that this action may help explain the synaptic changes seen in Alzheimer's disease.
引用
收藏
页码：2409 / 2413
页数：5
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