A METHOD FOR ELUTING DNA IN A WIDE-RANGE OF MOLECULAR-WEIGHTS FROM AGAROSE GELS

被引：8

作者：

DURO, G ^{[1
]}

IZZO, V ^{[1
]}

BARBIERI, R ^{[1
]}

CANTONE, M ^{[1
]}

COSTA, MA ^{[1
]}

GIUDICE, G ^{[1
]}

机构：

[1] CNR,DIPARTIMENTO BIOL CELLULARE,I-90123 PALERMO,ITALY

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90304-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple and rapid method for recovering DNAs of a wide range of molecular weights from agarose gels. A DNA-containing gel slice is placed on a Parafilm sheet in the center of a circular (positive) electrode and covered with a drop of buffer, while a linear (negative) electrode is placed on the top of the gel and driven about 1 mm into the gel itself. When a continuous current is applied, the DNA migrates into the buffer toward the circular electrode. We have obtained almost total recovery of DNAs up to 10 kb in size. Our method may also be used, under appropriate conditions, for higher molecular weight DNAs. The yield and all the biological assays performed on the DNAs obtained by our method recommend it for routine laboratory use. © 1991.

引用

页码：111 / 115

页数：5

共 21 条

[1] RECOVERY OF DNA SEGMENTS FROM AGAROSE GELS [J].