SIMPLE AND UNAMBIGUOUS METHOD FOR IDENTIFYING URINARY ACYLCARNITINES USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：40

作者：

LOWES, S ^{[1
]}

ROSE, ME ^{[1
]}

机构：

[1] OPEN UNIV,DEPT CHEM,MILTON KEYNES MK7 6AA,BUCKS,ENGLAND

来源：

ANALYST | 1990年 / 115卷 / 05期

关键词：

Acylcarnitine; Chemical derivatisation; Clinical analysis; Gas chromatography - Mass spectrometry; Urine;

D O I：

10.1039/an9901500511

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Several inherited metabolic disorders, particularly the organic acidurias and acidemias, are often characterised by excretion of acylcarnitines, especially octanoylcarnitine, in the urine. Clinical investigation of such serious disorders ideally requires a rapid, simple and selective method for determining acylcarnitines in urine. Initial results are given here of a method that may approach this ideal. The procedure involves chemical derivatisation, in which the zwitterionic acylcarnitines are cyclised to volatile lactones, and analysis by gas chromatography and gas chromatography - mass spectrometry. Preparation of urine samples by ion-exchange purification and an illustrative application of the proposed method to a clinical sample are also outlined.

引用

页码：511 / 516

页数：6

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