Solid-state fermentation for the production of biomass valorizing feruloyl esterase

被引:7
作者
Gopalan, Nishant [1 ,2 ]
Nampoothiri, Kesavan Madhavan [1 ]
Szakacs, George [3 ]
Parameswaran, Binod [1 ]
Pandey, Ashok [1 ]
机构
[1] CSIR Natl Inst Interdisciplinary Sci & Technol, Div Biotechnol, Thiruvananthapuram, Kerala, India
[2] CSIR, Acad Sci & Innovat Res AcSIR, New Delhi, India
[3] Budapest Univ Technol & Econ, Dept Appl Biotechnol & Food Sci, Geller Ter 4, H-1111 Budapest, Hungary
关键词
Feruloyl esterase; Aspergillus niger; Bioprocess; Solid state fermentation (SSF); AnFaeA;
D O I
10.1016/j.bcab.2016.04.009
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aspergillus niger ATCC 13497 has been used to produce feruloyl esterase type A enzyme. The process parameters were optimized to attain a maximum of 11984.1 U/gds of enzyme activity within a period of 72 h using destarched wheat bran as a substrate. The purified enzyme studied in the present work displays a pH optimum of 5, and a temperature optimum of 37 degrees C. The enzyme has a size of 35 kDa and exhibited moderate thermostability, with 41% of activity retained at 90 degrees C. Ni (+2) and Co (+2) ions were found to have stimulatory activity on the enzyme with 66% and 78% increase in activity respectively. Cu+2 inhibited the activity by 88%. Statistical optimization enhanced the enzyme activity by 2.47 fold. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7 / 13
页数:7
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