Identification of Potential Plasma Biomarkers for Abdominal Aortic Aneurysm Using Tandem Mass Tag Quantitative Proteomics

被引:12
作者
Henriksson, Anders E. [1 ,2 ,3 ]
Lindqvist, Markus [1 ]
Sihlbom, Carina [4 ]
Bergstrom, Jorgen [4 ]
Bylund, Dan [3 ]
机构
[1] Sundsvall Cty Hosp, Dept Lab Med, SE-85186 Sundsvall, Sweden
[2] Sundsvall Cty Hosp, Dept Surg, SE-85186 Sundsvall, Sweden
[3] Mid Sweden Univ, Dept Nat Sci, SE-85170 Sundsvall, Sweden
[4] Univ Gothenburg, Prote Core Facil, SE-40530 Gothenburg, Sweden
关键词
aortic aneurysm; biomarker; bleomycin hydrolase; clinical proteomics; mass spectrometry; proteomics;
D O I
10.3390/proteomes6040043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative proteomic approach to find new, potential plasma AAA biomarker candidates. Pre-fractionated plasma samples from twelve patients with AAA and eight matched controls without aneurysm were analyzed by mass spectrometry applying a tandem mass tag (TMT) technique. Eight proteins were differentially regulated in patients compared to controls, including decreased levels of the enzyme bleomycin hydrolase. The down-regulation of this enzyme was confirmed in an extended validation study using an enzyme-linked immunosorbent assay (ELISA). The TMT-based proteomic approach thus identified novel potential plasma biomarkers for AAA.
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页数:9
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