HUMAN PLATELET ANTIGEN-5 (BR) GENOTYPING BY ASPA - ALLELE-SPECIFIC PRIMER AMPLIFICATION (PCR-SSP)

被引：17

作者：

SIMSEK, S

BLEEKER, PMM

HEEREMANS, J

VONDEMBORNE, AEGK

机构：

[1] UNIV AMSTERDAM,EXPTL & CLIN IMMUNOL LAB,AMSTERDAM,NETHERLANDS

[2] NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,1006 AK AMSTERDAM,NETHERLANDS

[3] UNIV AMSTERDAM,ACAD MED CTR,DEPT HAEMATOL,1105 AZ AMSTERDAM,NETHERLANDS

[4] UNIV AMSTERDAM,ACAD MED CTR,CTR BLOOD CELL RES,1105 AZ AMSTERDAM,NETHERLANDS

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1994年 / 88卷 / 03期

关键词：

GLYCOPROTEIN IA; PLATELET ALLOANTIGENS; IMMUNE-MEDIATED THROMBOCYTOPENIAS; HPA-5; GENOTYPE; ASPA;

D O I：

10.1111/j.1365-2141.1994.tb05096.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have developed a PCR assay named ASPA (allele-specific primer amplification) to determine the HPA-5a and -5b genotypes. It consists of two PCR-reactions. One primer of each primer set has a 3'-end nucleotide which is specific for A or G at position 505 of the HPA-5b or -5a allele respectively. The HPA-5 genotypes determined in this way were strictly concordant with the genotypes established by PCR-ASRA and with the phenotypes established using MAIPA. The ASPA is a rapid and reliable technique and can be used for the determination of alleles which code for platelet antigen allotypes.

引用

页码：659 / 661

页数：3

共 11 条

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BORNE AEG, 1994, IMMUNOBIOLOGY TRANSF, P149

[2]

BORNE AEG, 1989, LEUCOCYTE TYPING, V4

[3]

CIULLA TA, 1988, ANAL BIOCHEM, V174, P458

[4] INTEGRINS - A FAMILY OF CELL-SURFACE RECEPTORS [J].