Cell Synchronization by Double Thymidine Block

被引：123
作者：
Chen, Guo
Deng, Xingming [1 ]
机构：
[1] Emory Univ, Sch Med, Dept Radiat Oncol, Atlanta, GA 30322 USA
来源：
BIO-PROTOCOL | 2018年 / 8卷 / 17期
关键词：
Cell synchronization; Cell cycle; Thymidine; DNA synthesis; DNA replication;
D O I：
10.21769/BioProtoc.2994
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.
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页数：5
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