GENE-TRANSFER STRATEGIES IN PLANTS

被引:16
作者
SAWAHEL, WA
COVE, DJ
机构
[1] Genetics Department, Leeds University, Leeds
关键词
NATURAL TRANSFORMATION SYSTEMS; ELECTROPORATION; ELECTROINJECTION; ELECTROPHORESIS; PROTOPLAST FUSION; MACROINJECTION; MICROINJECTION; LIPOSOME SYSTEM; CHEMICAL METHODS; POLLEN TRANSFORMATION; UV LASER MICROBEAM; PARTICLE GUN; GENOMIC DNA;
D O I
10.1016/0734-9750(92)90302-P
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In recent years, a large number of gene transfer methods have been developed. However, the results of these studies have often been published in such a way that it has been extremely difficult for researchers to assess the reliability and efficiency of the method, and to judge whether or not integrative transformation has occurred. Thus although an abundance of knowledge exists within the area of gene transfer, its documentation remains disjointed. This report summarises the recent progress which has been made in the field of gene transfer systems in plants and discusses the associated advantages, disadvantages and limitations in an attempt to clarify this issue.
引用
收藏
页码:393 / 412
页数:20
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