CORRELATION BETWEEN APOPTOSIS, TUMORIGENESIS, AND LEVELS OF INSULIN-LIKE GROWTH-FACTOR-I RECEPTORS

We have investigated whether there is a quantitative relationship between the insulin-like growth factor I receptor (IGF-IR), the extent of apoptosis in vivo, and tumorigenesis. C6 rat glioblastoma cells were treated with increasing concentrations of antisense oligodeoxynucleotides to the IGF-IR RNA. The extent of apoptosis in vivo is correlated to the decrease in EGF-IR levels and, in turn, tumorigenesis in nude mice is correlated to the fraction of surviving cells, In syngeneic rats, a host response leads to complete inhibition of tumorigenesis, These findings establish, for the first time on a quantitative basis, the relationship between IGF-IR levels and the extent of apoptosis, as well as the relationship between the initial apoptotic event and the time of appearance of transplantable tumors.