DIFFERENTIATION OF HEPATIC AND HEMATOPOIETIC CELLS AND SYNTHESIS OF BLOOD SERUM PROTEINS IN ORGAN CULTURES OF LIVER

In organ cultures of embryonal liver on millipore filters prolonged growth and differentiation of hepatic and hemopoietic tissues was obtained. This was manifested both morphologically and by specific syntheses-of serum albumin and of α-F-globulin. Adult liver of mice treated by CCl4 12-72 h before explantation acquired the capacity to grow in organ cultures and to secrete serum albumin in the culture medium. This capacity seems to correlate with the degree of lymphoid infiltration of the liver tissue prior to explantation. © 1969.