PREPARATION OF D-PSICOSE FROM D-FRUCTOSE BY IMMOBILIZED D-TAGATOSE 3-EPIMERASE

被引:109
|
作者
ITOH, H [1 ]
SATO, T [1 ]
IZUMORI, K [1 ]
机构
[1] KAGAWA UNIV,FAC AGR,DEPT BIORESOURCE SCI,KAGAWA 76107,JAPAN
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 80卷 / 01期
关键词
D-TAGATOSE; 3-EPIMERASE; PSEUDOMONAS SP ST-24; D-XYLOSE ISOMERASE; D-PSICOSE PREPARATION; D-FRUCTOSE; D-GLUCOSE;
D O I
10.1016/0922-338X(95)98186-O
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
D-Tagatose 3-epimerase (D-TE) from Pseudomonas sp. ST-24 immobilized on Chitopearl beads of BCW 2503 was used in the preparation of D-psicose from D-fructose. When D-fructose solution (10%) was passed through a column packed with immobilized D-TE about 20% of the D-fructose was converted to D-psicose. This column was continuously used for 10 d at 45 degrees C and about 90 g of D-psicose were obtained from D-fructose (500 g). Through the coupling with immobilized D-TE and D-xylose isomerase, D-psicose could be prepared directly from D-glucose.
引用
收藏
页码:101 / 103
页数:3
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