ENZYME ELECTRODE WITH COLLAGEN-IMMOBILIZED CHOLESTEROL OXIDASE FOR THE MICRO-DETERMINATION OF FREE-CHOLESTEROL

被引：35

作者：

BERTRAND, C

COULET, PR

GAUTHERON, DC

机构：

[1] Laboratoire de Biologie et Technologie des Membranes du CNRS, Universite Claude Bernard, 69622, Villeurbanne, Lyon I 43

来源：

ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS | 1979年 / 12卷 / 14期

关键词：

Clinical Analysis; Collagen membrane; Enzymatic cholesterol; Enzyme Electrode;

D O I：

10.1080/00032717908082547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol oxidase has been immobilized on collagen films associated to an electrochemical sensor to form a “cholesterol electrode”. The electrode poised at a potential of + 650 my vs Ag/AgCl detects the hydrogen peroxide produced in the enzymatic reaction. This device presents a very high sensitivity and a wide range of linearity (10 -7 M - 0.8.10 -4 M). The use of a non enzymatic electrode associated with the enzymatic one allowed the detection and correction of electrochemical interferences when applied to human sera for free cholesterol determination. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：1477 / 1488

页数：12