PACKED-COLUMN SUBCRITICAL FLUID CHROMATOGRAPHY OF UNDERIVATIZED AMINO-ACIDS

被引：40

作者：

CAMEL, V

THIEBAUT, D

CAUDE, M

DREUX, M

机构：

[1] ECOLE SUPER PHYS & CHIM IND,CHIM ANALYT PROC IND LAB,10 RUE VAUQUELIN,F-75231 PARIS 05,FRANCE

[2] UNIV ORLEANS,CHIM ANALYT LAB,ORLEANS 2,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 605卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85032-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Packed column subcritical fluid chromatography of underivatized amino acids is described. Using pyridine (or ethylene glycol)-methanol-water-triethylamine as modifier in the carbon dioxide, mixtures of amino acids can be separated on diol-bonded silica and detected without derivatization using evaporative light-scattering detection. Pyridine and ethylene glycol are shown to impregnate the stationary phase and to improve efficiency. The results demonstrate the wide potential of packed column subcritical fluid chromatography for the determination of polar compounds.

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页码：95 / 101

页数：7

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