REPLICATION OF ADENOVIRUS DNA IN-VITRO IS ATP-INDEPENDENT

被引：11
|
作者：
PRONK, R [1 ]
VANDRIEL, W [1 ]
VANDERVLIET, PC [1 ]
机构：
[1] UNIV UTRECHT,PHYSIOL CHEM LAB,3521 GG UTRECHT,NETHERLANDS
来源：
FEBS LETTERS | 1994年 / 337卷 / 01期
关键词：
ADENOVIRUS; ATP; DNA POLYMERASE; DNA REPLICATION; DIVALENT CATION;
D O I：
10.1016/0014-5793(94)80624-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10-fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+ could efficiently be used for DNA chain elongation on synthetic templates we hypothesize that Mg2+ is essential for the transition of initiation to elongation.
引用
收藏
页码：33 / 38
页数：6
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