MOLECULAR DIVERSITY OF COMMON BEAN (PHASEOLUS VULGARIS L.) CULTIVARS

被引：0

作者：

Filimon, Raluca ^{[1
]}

Nechifor, B. ^{[1
]}

Szilagyi, Lizica ^{[1
]}

机构：

[1] Univ Agron Sci & Vet Med Bucharest, Bucharest, Romania

来源：

SCIENTIFIC PAPERS-SERIES A-AGRONOMY | 2011年 / 54卷

关键词：

Phaseolus vulgaris; RAPD markers; genetic diversity;

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RAPD markers were used to evaluate genetic diversity among 8 common bean cultivars (Phaseolus vulgaris L.) that included six developed in Romania and two in CIAT Colombia. Only 4 of all the 8 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterization of bean cultivars. These four primers (OPD-08, OPG-03, OPG-12, OPY-20) generated 33 DNA bands, of which 17 bands showing polymorphism (48.84%). On average, each primer generated 8.25 bands, of which 4.25 were polymorphic. Genetic distances were calculated using Nei&Li (1979) similarity coefficient, displayed in a dendrogram (UPGMA method). Cluster analysis based on RAPD amplification products divided genotypes in two main groups, according to their geographical origin. Genetic similarity vary greatly (60% - 96%), depending on the pairs of genotypes and on the groups.

引用

页码：344 / 349

页数：6

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