IDENTIFICATION OF A GEPHYRIN BINDING MOTIF ON THE GLYCINE RECEPTOR-BETA SUBUNIT

被引：350

作者：

MEYER, G ^{[1
]}

KIRSCH, J ^{[1
]}

BETZ, H ^{[1
]}

LANGOSCH, D ^{[1
]}

机构：

[1] UNIV FRANKFURT,ZENTRUM MORPHOL,D-60590 FRANKFURT,GERMANY

来源：

NEURON | 1995年 / 15卷 / 03期

关键词：

D O I：

10.1016/0896-6273(95)90145-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GlyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GlyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the beta subunit. GlyR alpha subunits and GABA(A) receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GlyR beta subunit into the GABA(A) receptor beta 1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that beta subunit expression is essential for the formation of a postsynaptic GlyR matrix.

引用

页码：563 / 572

页数：10

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