COMPARISON OF SOLUBLE AND MEMBRANE-BOUND PYROGLUTAMYL PEPTIDASE-I ACTIVITIES IN RAT-BRAIN TISSUES IN THE PRESENCE OF DETERGENTS

被引：11

作者：

ALBA, F ^{[1
]}

ARENAS, JC ^{[1
]}

LOPEZ, MA ^{[1
]}

机构：

[1] UNIV GRANADA,SCH MED,FEDERICO OLORIZ INST NEUROSCI,E-18012 GRANADA,SPAIN

来源：

NEUROPEPTIDES | 1995年 / 29卷 / 02期

关键词：

D O I：

10.1016/0143-4179(95)90092-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Pyroglutamyl peptidase I activity from soluble and membrane-bound fractions of rat brain homogenates is inhibited by the presence of sodium deoxycholate but not by triton X-100. Biobeads SM2, a polystyrene adsorbent reported to be useful in removing detergents from aqueous solutions, inhibits enzymatic activity in both fractions regardless of the presence of these detergents, probably because of partial adsorption of the enzyme by the polymeric microspheres. These effects seem to be enzyme-specific since other aminopeptidase activities are not affected by detergents or biobeads. The results suggest that soluble and membrane-bound forms of the enzyme represent the same protein in two different cell compartments.

引用

页码：103 / 107

页数：5

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