KINETIC AND IMMUNOPHENOTYPIC HETEROGENEITY IN ACUTE MYELOID-LEUKEMIA SUBPOPULATIONS

被引：0

作者：

LONGO, R ^{[1
]}

VECCHI, A ^{[1
]}

MESSORA, C ^{[1
]}

SACCHI, S ^{[1
]}

机构：

[1] UNIV MODENA,MED CLIN 2,I-41100 MODENA,ITALY

来源：

LEUKEMIA & LYMPHOMA | 1992年 / 6卷 / 02期

关键词：

AML; IMMUNOPHENOTYPE; 3H-TDR; CELL PROLIFERATION;

D O I：

10.3109/10428199209064890

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Bone marrow samples of 27 cases of acute myeloid leukemia were studied by double staining with 3H-Thymidine and monoclonal antibodies (APAAP method). 3H autoradiography labels the nucleus of S-phase cells and immunocytochemistry (APAAP) detects cytoplasmic antigens. Different myeloid subsets were characterized with the monoclonal antibodies My7 (CD13), My9 (CD33), My4 (CD14), IOM11c (CD11c), Ber Mac3 and anti-HLA-DR. We have shown that there are different myeloid subsets with different proliferative patterns. The most notable finding was that in M4 acute leukemia only myeloid subsets were in the S-phase, while in M5 acute leukemia the highest percentage of proliferating cells was in the monocytic subsets. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：161 / &

共 29 条

[1]

Raza A., Makeshuvari Y., Preisler H., Differences in cell cycle characteristic among patients with acute non lymphocytic leukemia, Blood, 69, pp. 1647-1653, (1987)

[2]

Hart J.S., George S.L., Frei E., Bodey G.P., Nickerson R.C., Freireich E.J., Prognostic significance of pretreatment proliferative activity in adult leukemia, Cancer, 39, (1977)

[3]

Colly L.P., Peters W.G., Hermans J., Arentsen-Honolers W., Willemze R., Percentage of S-phase cells in bone marrow aspirates, biopsy specimens and bone marrow aspirates corrected for blood dilution from patients with acute leukemia, Leuk. Res, 11, pp. 209-214, (1987)

[4]

Raza A., Yasin Z., Grande S., A comparison of the rate of DNA synthesis in myeloblasts from peripheral blood and bone marrows of patients with acute non lymphocytic leukemia, Exp. Cell, Res, 176, pp. 13-19, (1988)

[5]

Riccardi A., Danova M., Montecucco C., Ucci G., Cassano E., Giordano M., Mazzini G., Giordano P., Adult acute non lymphoblastic leukemia: reliability and prognostic significance of pretreatment bone marrow S-phase size determined by flow cytometry 1986, Scand. J. Haemat, 36, pp. 11-17, (1986)

[6]

Bennet J.M., Catovsky D., Daniel M.T., Flandrin G., Galton D.A.G., Gralnick H.R., Sultan C., Proposals for the classification of acute leukemias, Br. J. Haemat, 33, pp. 451-458, (1976)

[7]

Bennet J.M., Catovsky D., Daniel M.T., Flandrin G., Galton D.A.G., Gralnick H.R., Sultan C., Criteria for the diagnosis of acute leukemia of megakaryocyte lineage, Ann. Intern. Med, 103, pp. 460-465, (1985)

[8]

Bennet J.M., Catovsky D., Daniel M.T., Flandrin G., Galton D.A.G., Gralnick H.R., Sultan C., Proposed revised criteria for the classification of acute myeloid leukemia, Ann. Intern. Med, 103, pp. 626-632, (1985)

[9]

Boyum A., Separation of leukocytes from blood and bone marrow, Scand. J. Clin. Lab. Invest, 21, (1968)

[10]

Cordell J.L., Falini B., Erber W.N., Ghosh A.K., Abdulaziz Z., McDonald S., Pulford K.A.F., Stein H., Mason D.Y., Immunoenzymatic labelling of monoclonal antibodies using immunocomplexes of alkaline phosphatase and monoclonal anti alkaline phosphatase (APAAP complexes), J. Histochem. Cytochem, 32, pp. 219-225, (1984)

← 1 2 3 →