THE EFFECT OF INTRAVENOUS RECOMBINANT HUMAN RENIN ON BLOOD-PRESSURE IN PITHED SPONTANEOUSLY HYPERTENSIVE RATS

被引：1

作者：

SHIBASAKI, M ^{[1
]}

SUDOH, K ^{[1
]}

ASANO, M ^{[1
]}

MURAKAMI, K ^{[1
]}

机构：

[1] UNIV TSUKUBA, INST APPL BIOCHEM, TSUKUBA, IBARAKI 305, JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1992年 / 215卷 / 2-3期

关键词：

RECOMBINANT RENIN (HUMAN); SPONTANEOUSLY HYPERTENSIVE RAT (SHR); BLOOD PRESSURE; RENIN INHIBITORS;

D O I：

10.1016/0014-2999(92)90038-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of highly purified recombinant human renin (rh-renin), expressed in Chinese hamster ovary cells, on mean blood pressure (MBP) was evaluated in pithed spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Intravenous bolus injection of rh-renin produced dose-dependent increases in MBP in pithed SHR and WKY. The pressor response to rh-renin in pithed SHR was about 3 times as potent as that in pithed WKY. Intravenous infusion of rh-renin produced dose-dependent progressive increases in MBP during the first 40 min, reaching plateaus and thereafter MBP was maintained up to 120 min. This hypertensive response to rh-renin was antagonized by renin inhibitors, YM-21095 and KRI-1314, which inhibited the reaction between rh-renin and tetradecapeptide competitively, with K(i) values of 5.1 x 10(-10) and 4.3 x 10(-9) M, respectively. In rh-renin-infused pithed SHR, the hypotensive effect of YM-21095 was 37 times as potent as that of KRI-1314. These results suggest that rh-renin can stimulate the rat renin-angiotensin system, thereby producing hypertension. Moreover, the rh-renin-infused rat model could be useful to evaluate the effect of renin inhibitor.

引用

页码：271 / 276

页数：6

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