AN IGM SPECIFIC ELISA FOR THE SERODIAGNOSIS OF VIRAL BOVINE RESPIRATORY-INFECTIONS

被引：6
作者：
FLORENT, G
WISEMAN, A
机构：
来源：
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 1990年 / 13卷 / 04期
关键词：
DIAGNOSTIC ELISA; IGM; IBR; BRSV;
D O I：
10.1016/0147-9571(90)90089-C
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A capture ELISA for the detection of IgM antibodies to Infectious Bovine Rhinotracheitis (IBR) and to Bovine Respiratory Syncytial (BRS) viruses was developed. In these assays, the first monoclonal antibody to bovine IgM is used as the catching antibody while the second monoclonal detects specific antiviral antibodies. The test was evaluated on serum samples originating from both experimentally and naturally infected animals. From these studies, it has been shown that primary IBR and BRS virus infections can be confirmed using serum samples collected 5-10 days after the appearance of the clinical signs of disease.
引用
收藏
页码：203 / 208
页数：6
相关论文
共 10 条
[1]   AN EVALUATION OF 5 SEROLOGICAL TESTS FOR THE DETECTION OF ANTIBODY TO BOVINE HERPESVIRUS-1 IN VACCINATED AND EXPERIMENTALLY INFECTED CATTLE [J].
EDWARDS, S ;
WOODS, SB ;
WESTCOTT, DG ;
EMMERSON, M ;
JONES, PC ;
PHILLIPS, AJ .
RESEARCH IN VETERINARY SCIENCE, 1986, 41 (03) :378-382
[2]   ENZYME-LINKED-IMMUNOSORBENT-ASSAY USED TO MONITOR SERUM ANTIBODIES TO BOVINE RESPIRATORY-DISEASE VIRUSES [J].
FLORENT, G ;
DEMARNEFFE, C .
VETERINARY MICROBIOLOGY, 1986, 11 (04) :309-317
[3]   ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR SINGLE SERUM DIAGNOSIS OF CANINE PARVOVIRUS DISEASE [J].
FLORENT, G .
VETERINARY RECORD, 1986, 119 (19) :479-480
[4]   EPIDEMIOLOGICAL-STUDY OF BOVINE RESPIRATORY SYNCYTIAL VIRUS-INFECTIONS IN CALVES - INFLUENCE OF MATERNAL ANTIBODIES ON THE OUTCOME OF DISEASE [J].
KIMMAN, TG ;
ZIMMER, GM ;
WESTENBRINK, F ;
MARS, J ;
VANLEEUWEN, E .
VETERINARY RECORD, 1988, 123 (04) :104-109
[5]   ISOTYPE-SPECIFIC ELISAS FOR THE DETECTION OF ANTIBODIES TO BOVINE RESPIRATORY SYNCYTIAL VIRUS [J].
KIMMAN, TG ;
WESTENBRINK, F ;
STRAVER, PJ ;
VANZAANE, D ;
SCHREUDER, BEC .
RESEARCH IN VETERINARY SCIENCE, 1987, 43 (02) :180-187
[6]  
LETESSON JJ, 1985, ANN MED VET, V129, P131
[7]  
OSARIO F, 1989, J VET DIAGN INVEST, V1, P139
[8]  
POTGIETER LND, 1977, AM J VET RES, V38, P1341
[9]  
WESTENBRINK F, 1986, AM J VET RES, V48, P1133
[10]  
Zygraich N., 1982, 12TH P WORLD C DIS C, P189
1