Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

被引：15

作者：

Laggai, Stephan ^{[1
]}

Simon, Yvette ^{[1
]}

Ranssweiler, Theo ^{[1
]}

Kiemer, Alexandra K. ^{[1
]}

Kessler, Sonja M. ^{[1
]}

机构：

[1] Saarland Univ, Dept Pharm Pharmaceut Biol, D-66123 Saarbrucken, Germany

来源：

WORLD JOURNAL OF HEPATOLOGY | 2013年 / 5卷 / 10期

关键词：

Non alcoholic steatohepatitis; Non alcoholic fatty liver disease; Thin layer chromatography; IMP2/IGF2BP2; p62; Methionine choline deficient diet; Polar lipids; neutral lipids; Phosphatidylcholine/phosphatidylethanolamine ratio; Triglycerides;

D O I：

10.4254/wjh.v5.i10.558

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To establish a simple method to quantify lipid classes in liver diseases and to decipher the lipid profile in p62/IMP2-2/IGF2BP2-2 transgenic mice. METHODS: Liver-specific overexpression of the insulin-like growth factor 2 mRNA binding protein p62/IMP2-2/IGF2BP2-2 was used as a model for steatosis. Steatohepatitis was induced by feeding a methioninecholine deficient diet. Steatosis was assessed histologically. For thin layer chromatographic analysis, lipids were extracted from freeze-dried tissues by hexane/2-propanol,dried, redissolved, and chromatographically separated by a two-solvent system. Dilution series of lipid standards were chromatographed, detected, and quantified. The detection was performed by either 2',7'-dichlorofluoresceine or a sulfuric acid/ethanol mixture. RESULTS: Histological analyses confirmed steatosis and steatohepatitis development. The extraction, chromatographic, and detection method showed high inter-assay reproducibility and allowed quantification of the different lipid classes. The analyses confirmed an increase of triglycerides and phosphatidylethanolamine and a decrease in phosphatidylcholine in the methionine-choline deficient diet. The method was used for the first time to asses the lipid classes induced in the p62-overexpressing mouse model and showed a significant increase in all detected lipid species with a prominent increase of triglycerides by 2-fold. Interestingly, the ratio of phosphatidylcholine to phosphatidylethanolamine was decreased, as previously suggested as a marker in the progression from steatosis to steatohepatitis. CONCLUSION: The thin layer chromatography analysis allows a reliable quantification of lipid classes and provides detailed insight into the lipogenic effect of p62. (C) 2013 Baishideng. All rights reserved.

引用

页码：558 / 567

页数：10

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