A CONSTANT RADIUS OF CURVATURE MODEL FOR THE ORGANIZATION OF DNA IN TOROIDAL CONDENSATES

被引:156
作者
HUD, NV [1 ]
DOWNING, KH [1 ]
BALHORN, R [1 ]
机构
[1] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV LIFE SCI,BERKELEY,CA 94720
关键词
PACKAGING; BACTERIOPHAGE; PSI DNA;
D O I
10.1073/pnas.92.8.3581
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Toroidal DNA condensates have received considerable attention for their possible relationship to the packaging of DNA in viruses and in general as a model of ordered DNA condensation. A spool-like model has primarily been supported for DNA organization within toroids. However, our observations suggest that the actual organization may be considerably different. We present an alternate model in which DNA for a given toroid is organized within a series of equally sized contiguous loops that precess about the toroid axis. A related model for the toroid formation process is also presented. This kinetic model predicts a distribution of toroid sizes for DNA condensed from solution that is in good agreement with experimental data.
引用
收藏
页码:3581 / 3585
页数:5
相关论文
共 25 条
[1]   STRUCTURE OF VIRAL PSI-29 DNA CONDENSED BY SIMPLE TRIAMINES - A LIGHT-SCATTERING AND ELECTRON-MICROSCOPY STUDY [J].
ALLISON, SA ;
HERR, JC ;
SCHURR, JM .
BIOPOLYMERS, 1981, 20 (03) :469-488
[2]   CONDENSATION OF DNA BY TRIVALENT CATIONS .1. EFFECTS OF DNA LENGTH AND TOPOLOGY ON THE SIZE AND SHAPE OF CONDENSED PARTICLES [J].
ARSCOTT, PG ;
LI, AZ ;
BLOOMFIELD, VA .
BIOPOLYMERS, 1990, 30 (5-6) :619-630
[3]   CONDENSATION OF DNA BY MULTIVALENT CATIONS - CONSIDERATIONS ON MECHANISM [J].
BLOOMFIELD, VA .
BIOPOLYMERS, 1991, 31 (13) :1471-1481
[4]   DNA CONDENSATION WITH POLYAMINES .2. ELECTRON-MICROSCOPIC STUDIES [J].
CHATTORAJ, DK ;
GOSULE, LC ;
SCHELLMAN, JA .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 121 (03) :327-337
[5]   DNA ARRANGEMENT IN ISOMETRIC PHAGE HEADS [J].
EARNSHAW, WC ;
HARRISON, SC .
NATURE, 1977, 268 (5621) :598-602
[6]   DNA CONDENSATION WITH POLYAMINES .1. SPECTROSCOPIC STUDIES [J].
GOSULE, LC ;
SCHELLMAN, JA .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 121 (03) :311-326
[7]  
GOSULE LC, 1976, NATURE, V259, P333, DOI 10.1038/259333a0
[8]  
HAGERMAN PJ, 1988, ANNU REV BIOPHYS BIO, V17, P265
[9]   STRUCTURE OF NUCLEIC ACID-POLY BASE COMPLEXES [J].
HAYNES, M ;
GARRETT, RA ;
GRATZER, WB .
BIOCHEMISTRY, 1970, 9 (22) :4410-&
[10]   STRUCTURE OF HISTONE H1-DNA COMPLEX - EFFECT OF HISTONE H-1 ON DNA CONDENSATION [J].
HSIANG, MW ;
COLE, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :4852-4856