Wheat doubled haploid (DH) lines were produced from the F1 hybrid, Fukuho-komugi x Oligo Culm, through intergeneric crosses between wheat and maize. F2 plants and 203 DH lines were analyzed for the segregation of the eight genetic markers, namely, grain proteins, grain esterases, GA-insensitivity and glume traits. The segregation in the F2 plants fitted to the expected ratios. No deviation was observed among the DH lines, either, except for the glume pubescence. The result indicate-s the absence of correlation between the markers investigated and the efficiency of embryo formation in the DH lines.