MONOCLONAL ANTIBODIES REACTING WITH MURINE TERATOCARCINOMA CELLS

被引：48
作者：
GOODFELLOW, PN
LEVINSON, JR
WILLIAMS, VE
MCDEVITT, HO
机构：
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 01期
关键词：
D O I：
10.1073/pnas.76.1.377
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a rat immunized with the C3H mouse teratocarcinoma C86-SI. After the fusion two clones were chosen for further analysis. The first clone, 3C4-10, produced an antibody recognizing an antigen with a distribution restricted to teratocarcinoma cell lines, an endoderm cell line, and a neuroblastoma. The second clone, 4A1-9, produced an antibody that reacted with all cultured murine cells tested and adult brain. Neither antibody reacted with preimplantation embryos. The 3C4-10 antibody recognized an antigen associated with proteins. The apparent molecular weight of the 3C4-10 antigen was greater than 100,000.
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页码：377 / 380
页数：4
相关论文
共 34 条
[1]  
ACTON RT, 1974, EUR J IMMUNOL, V4, P498
[2]   SURFACE ANTIGENS COMMON TO MOUSE CLEAVAGE EMBRYOS AND PRIMITIVE TERATOCARCINOMA CELLS IN CULTURE [J].
ARTZT, K ;
DUBOIS, P ;
BENNETT, D ;
CONDAMINE, H ;
BABINET, C ;
JACOB, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (10) :2988-2992
[3]   EMBRYONIC SURFACE-ANTIGENS - QUASI-ENDODERMAL TERATOMA ANTIGEN [J].
ARTZT, K ;
HAMBURGER, L ;
JAKOB, H ;
JACOB, F .
DEVELOPMENTAL BIOLOGY, 1976, 51 (01) :152-157
[4]   ESTABLISHMENT OF FUNCTIONAL CLONAL LINES OF NEURONS FROM MOUSE NEUROBLASTOMA [J].
AUGUSTIT.G ;
SATO, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 64 (01) :311-&
[5]  
BENNETT D, 1971, 3 P LEP COLL LOND, P247
[6]  
BERNSTINE EG, 1973, P NATL ACAD SCI US, V70, P2899
[7]  
COTTON RGH, 1973, EUR J IMMUNOL, V3, P136
[8]   CELL-SURFACE ANTIGENS OF TOTIPOTENT MOUSE TERATOCARCINOMA CELLS GROWN INVIVO - THEIR RELATION TO EMBRYO, ADULT, AND TUMOR ANTIGENS [J].
DEWEY, MJ ;
GEARHART, JD ;
MINTZ, B .
DEVELOPMENTAL BIOLOGY, 1977, 55 (02) :359-374
[9]   PCC4, A NEW CELL-SURFACE ANTIGEN COMMON TO MULTIPOTENTIAL EMBRYONAL CARCINOMA CELLS, SPERMATOZOA, AND MOUSE EARLY EMBRYOS [J].
GACHELIN, G ;
KEMLER, R ;
KELLY, F ;
JACOB, F .
DEVELOPMENTAL BIOLOGY, 1977, 57 (01) :199-209
[10]   ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES [J].
GALFRE, G ;
HOWE, SC ;
MILSTEIN, C ;
BUTCHER, GW ;
HOWARD, JC .
NATURE, 1977, 266 (5602) :550-552
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