KIH-201 - A NEW ENHANCER OF CHEMILUMINESCENCE IN THE LUMINOL-HYDROGEN PEROXIDE-PEROXIDASE SYSTEM CHARACTERIZED BY A HIGH-PERFORMANCE LUMINOMETER

被引：12

作者：

HORI, H ^{[1
]}

FUJII, T ^{[1
]}

KUBO, A ^{[1
]}

PAN, N ^{[1
]}

SAKO, S ^{[1
]}

TADA, C ^{[1
]}

MATSUBARA, T ^{[1
]}

机构：

[1] TEIKOKU SEIYAKU CO LTD,KAGAWA 76926,JAPAN

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 06期

关键词：

SYNTHETIC PHENOL; ENHANCER; CHEMILUMINESCENCE; HORSERADISH PEROXIDASE; LUMINOL; HYDROGEN PEROXIDE;

D O I：

10.1080/00032719408000282

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We developed new phenolic enhancers of chemiluminescence in the luminol-hydrogen peroxide-horseradish peroxidase reaction. We designed, synthesized, and tested three series of. phenolic enhancers as follows: 1) hydroxybenzylidenc-cyclopentenediones (KIH-201, KIH-203, TX-1123, and TX-1124), 2) 4-hydroxybenzylidene-malononitriles (TX-825, TX-1121, TX-1122, TX-1840 and SF-6847), and 3) 2, 4-dichlorophenol derivative (TX-816). Luminescence measurements were performed with a new high-performance luminometer, which have a superior photon counting system (gate time: 10-100 ms) and an automatic injector (variable range: 10-190 mu l). We found that the arylidene-cyclopentenedione KIH-201 [2-(4'-hydroxy3'-methoxy-benzylidene)-4-cyclopentene-1,3-dione] was an effective enhancer similar to that of p-iodophenol, known as the most strong enhancer of this chemiluminescent system. This KIH-201 will facilitate the luminol - hydrogen peroxide - horseradish peroxidase reaction

引用

页码：1109 / 1122

页数：14

共 7 条

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