ENZYMATIC DEGRADATION OF POLY(HYDROXYALKANOATES) BY A MARINE BACTERIUM

被引：106
作者：
MUKAI, K
YAMADA, K
DOI, Y
机构：
[1] Polymer Chemistry Laboratory, The Institute of Physical and Chemical Research, (RIKEN) 2-1 Hirosawa, Wako-shi, Saitama
来源：
POLYMER DEGRADATION AND STABILITY | 1993年 / 41卷 / 01期
关键词：
D O I：
10.1016/0141-3910(93)90066-R
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
A bacterium capable of degrading poly(hydroxyalkanoates) (PHA) was isolated from sea water and identified as Comamonas testosteroni. The strain C. testosteroni excreted an extracellular PHA depolymerase and grew on poly(3-hydroxybutyrate) [P(3HB)] as a sole carbon source. The PHA depolymerase was purified from the culture medium to electrophoretic homogeneity by hydrophobic column chromatography, and its molecular weight was determined to be about 50000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The optimum activity of the depolymerase in degrading P(3HB) was observed in the alkaline region between pH9.5 and 10.0. The enzymatic degradation of microbial copolyesters was studied by the weight loss (erosion) of solution-cast films of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate). The PHA depolymerase had a very high hydrophobicity to adhere to the surface of the polyesters.
引用
收藏
页码：85 / 91
页数：7
相关论文
共 28 条
[1]   OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].
ANDERSON, AJ ;
DAWES, EA .
MICROBIOLOGICAL REVIEWS, 1990, 54 (04) :450-472
[2]   STUDIES OF COMPOSITION AND CRYSTALLINITY OF BACTERIAL POLY(BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE) [J].
BLOEMBERGEN, S ;
HOLDEN, DA ;
HAMER, GK ;
BLUHM, TL ;
MARCHESSAULT, RH .
MACROMOLECULES, 1986, 19 (11) :2865-2871
[3]   EXTRACELLULAR POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE FROM PENICILLIUM-FUNICULOSUM - GENERAL-CHARACTERISTICS AND ACTIVE-SITE STUDIES [J].
BRUCATO, CL ;
WONG, SS .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 290 (02) :497-502
[4]   POLYMER SYNTHESIS BY MICROORGANISMS - TECHNOLOGY AND ECONOMICS [J].
BYROM, D .
TRENDS IN BIOTECHNOLOGY, 1987, 5 (09) :246-250
[5]   POLY-BETA-HYDROXYBUTTERSAURE ABBAUENDE BAKTERIEN UND EXOENZYM [J].
CHOWDHURY, AA .
ARCHIV FUR MIKROBIOLOGIE, 1963, 47 (02) :167-+
[6]  
DELAFIELD EF, 1965, J BACTERIOL, V90, P1455
[7]  
DOI Y, 1988, MACROMOLECULES, V21, P2722, DOI 10.1021/ma00187a012
[8]  
DOI Y, 1986, MACROMOLECULES, V19, P2860, DOI 10.1021/ma00165a033
[9]  
DOI Y, 1992, POLYM DEGRAD STABIL, V36, P173, DOI 10.1016/0141-3910(92)90154-W
[10]  
DOI Y, 1988, APPL MICROBIOL BIOT, V28, P330, DOI 10.1007/BF00268190
123