PREPARATION OF RIGHT-SIDE-OUT PLASMA-MEMBRANE VESICLES FROM PENICILLIUM-CYCLOPIUM - A CRITICAL-ASSESSMENT OF MARKERS

被引：9

作者：

UGALDE, UO ^{[1
]}

HERNANDEZ, A ^{[1
]}

GALINDO, I ^{[1
]}

PITT, D ^{[1
]}

BARNES, JC ^{[1
]}

WAKLEY, G ^{[1
]}

机构：

[1] UNIV EXETER, WASHINGTON SINGER LABS, DEPT BIOL SCI, EXETER EX4 4QG, ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷

关键词：

D O I：

10.1099/00221287-138-10-2205

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A plasma membrane fraction was obtained by the combined use of differential centrifugation and aqueous polymer two-phase partitioning techniques. Vanadate-inhibited ATPase and glucan synthase activities were highly enriched in this fraction, although the presence of ATPase activity which was not inhibited by vanadate, nitrate, molybdate, anyimycin A or azide was also detected. Other intracellular membrane marker activities were present at very low or undetectable levels. A further separation step using Percoll density gradient centrifugation resulted in the separation of a fraction which exclusively contained vanadate-inhibited ATPase activity, and was enriched with silicotungstic-acid-staining membrane material. Latency tests performed on the plasma membrane markers showed that the membrane vesicles were in the right-side-out orientation.

引用

页码：2205 / 2212

页数：8

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[21] Voltage-dependent Ca2+ uptake by right-side-out plasma membrane vesicles derived from maize shoots [J].

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[22] SURFACE-PROPERTIES OF RIGHT SIDE-OUT PLASMA-MEMBRANE VESICLES ISOLATED FROM BARLEY ROOTS AND LEAVES [J].

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[23] VOLTAGE-DEPENDENT CA2+ INFLUX INTO RIGHT-SIDE-OUT PLASMA-MEMBRANE VESICLES ISOLATED FROM WHEAT ROOTS - CHARACTERIZATION OF A PUTATIVE CA2+ CHANNEL [J].

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