ISOLATION AND NUCLEOTIDE-SEQUENCE OF THE RIBOSOMAL-PROTEIN S16-ENCODING GENE FROM ASPERGILLUS-NIDULANS

被引：4
作者：
BRADSHAW, RE
PILLAR, TM
机构：
[1] Leicester Biocentre, University of Leicester, Leicester
来源：
GENE | 1991年 / 108卷 / 01期
关键词：
RECOMBINANT DNA; FILAMENTOUS FUNGUS; INTRON;
D O I：
10.1016/0378-1119(91)90502-3
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A genomic clone has been isolated from Aspergillus nidulans which is homologous to the ribosomal (r) protein S 16-encoding gene of Saccharomyces cerevisiae (S16A) and the r-protein S 19-encoding gene of rat (S19). The amino acid (aa) sequences, deduced from nucleotide (nt) sequence analysis, show that in both cases more than 63% of the aa are conserved. The proposed A. nidulans r-protein S16 gene (rps16) differs from that of S. cerevisiae in that it occurs as a single copy in the haploid genome (rather than two copies as in yeast) and contains two putative introns (rather than one). The mRNA leader is long compared to many Aspergillus genes, commencing 293 nt upstream from the coding region, and contains an open reading frame of 13 codons.
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页码：157 / 162
页数：6
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