PAF-INDUCED ACTIVATION OF POLYPHOSPHOINOSITIDE-HYDROLYZING PHOSPHOLIPASE-C IN CEREBRAL-CORTEX

被引：26

作者：

CATALAN, RE ^{[1
]}

MARTINEZ, AM ^{[1
]}

ARAGONES, MD ^{[1
]}

FERNANDEZ, I ^{[1
]}

LOMBARDIA, M ^{[1
]}

MIGUEL, BG ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC QUIM,DEPT BIOQUIM & BIOL MOLEC 1,E-28040 MADRID,SPAIN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91643-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The action of platelet-activating factor (PAF) on phosphoinositide hydrolysis was studied in rat brain slices. PAF produced a significant increase of 32P incorporation into phosphoinositides and phosphatidic acid (PA), in a dose- and time-dependent manner. Concomitantly, an increase of inositol phosphates and diacylglycerol (DAG) production was observed. Both inositol bisphosphate (IP2) and inositol trisphosphate (IP3) were detected as early as 5 s and they returned immediately to basal levels; concommitantly, formation of inositol monophosphate (IP) was detected. These findings demonstrated that PAF causes a rapid hydrolysis of polyphosphoinositides in cerebral cortex by a phospholipase C-dependent mechanism followed by subsequent resynthesis. © 1992.

引用

页码：300 / 305

页数：6

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