HELIUM-NEON LASER IRRADIATION OF RAT-LIVER MITOCHONDRIA GIVES RISE TO A NEW SUBPOPULATION OF MITOCHONDRIA - ISOLATION AND 1ST BIOCHEMICAL-CHARACTERIZATION

被引：18

作者：

GRECO, M

PERLINO, E

PASTORE, D

GUIDA, G

MARRA, E

QUAGLIARIELLO, E

机构：

[1] CNR,CTR STUDIO MITOCONDRI & METABOL ENERGET,I-70126 BARI,ITALY

[2] UNIV BARI,DIPARTMENTO BIOCHIM & BIOL MOLEC,I-70126 BARI,ITALY

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1991年 / 10卷 / 1-2期

关键词：

MITOCHONDRIA; HE-NE LASER; ENZYME; RNA; DNA; ISOLATION;

D O I：

10.1016/1011-1344(91)80213-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An experiment was performed to isolate the small atypical mitochondria produced during the irradiation of normal mitochondria with an He-Ne laser. Rat liver mitochondria were irradiated with a low-power continuous-wave He-Ne laser (energy dose, 5 J cm-2), followed by isolation using a sucrose gradient. In the irradiated sample, two bands were observed, one corresponding to normal mitochondria and the other to atypical mitochondria. Certain biochemical features of the mitochondria were investigated: mitochondrial enzyme activity and the presence of DNA and RNA were demonstrated. Hybridization experiments carried out with labelled mitochondrial probes, containing the genes for cytochrome oxidase subunit I and 12S rRNA, confirmed the mitochondrial nature of the isolated RNA.

引用

页码：71 / 78

页数：8

共 18 条

[1]

BERGMEYER HU, 1963, METHOD ENZYMAT AN, P978

[2] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].