PHOSPHOLIPASE-D ACTIVITY IN HEXANE

被引：7
作者：
SIMPSON, TD
机构：
[1] Agricultural Research Service, U.S. Department of Agriculture, Plant Biochemistry Research, Northern Regional Research Center, Peoria, 616O4, IL
来源：
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1991年 / 68卷 / 03期
关键词：
ORGANIC SOLVENT ACTIVITY; PHOSPHOLIPASE-D; SOYBEANS;
D O I：
10.1007/BF02657764
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Phospholipase D converts phosphatidylcholine (PC) to phosphatidic acid (PA) at 65-degrees-C in water-saturated hexane. Presumably, the active site of the enzyme remains hydrated in the interior of a lipid micelle. Enzyme activity at elevated temperatures in a nonaqueous medium contrasts sharply with inactivation at high temperature in aqueous solution. Results demonstrate that nonhydratable phospholipids can be produced enzymatically under conditions comparable to those during oil extraction in commercial soybean processing.
引用
收藏
页码：176 / 178
页数：3
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