CLONING IN ESCHERICHIA-COLI AND PHYSICAL STRUCTURE OF HEPATITIS-B VIRION DNA

被引：185

作者：

CHARNAY, P

POURCEL, C

LOUISE, A

FRITSCH, A

TIOLLAIS, P

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 05期

关键词：

D O I：

10.1073/pnas.76.5.2222

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in E. coli. By use of EcoRI, Xho I, BglII, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and the 5' end of the short strand was mapped at a fixed position.

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页码：2222 / 2226

页数：5

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