ANALYSIS OF THE MOUSE TYROSINASE PROMOTER IN-VITRO AND IN-VIVO

被引：20
作者：
GANSS, R
SCHMIDT, A
SCHUTZ, G
BEERMANN, F
机构：
[1] SWISS INST EXPTL CANC RES,CH-1066 EPALINGES,SWITZERLAND
[2] GERMAN CANC RES CTR,DIV MOLEC BIOL CELL 1,D-69120 HEIDELBERG,GERMANY
来源：
PIGMENT CELL RESEARCH | 1994年 / 7卷 / 05期
关键词：
MELANOCYTES; PIGMENTATION; TRANSGENIC MICE; TYROSINASE;
D O I：
10.1111/j.1600-0749.1994.tb00627.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The restricted expression of the tyrosinase gene in cells producing pigment suggests the presence of cis-regulatory elements and trans-acting tissue-specific factors. Since 270 bp upstream of the transcriptional start site contain sufficient information for tissue-specific and developmentally regulated expression, we confined our analyses to this region. In this article, we discuss the recent results we have obtained on the regulation of the mouse tyrosinase gene expression demonstrating the existence of one negative and two positive-acting elements in vitro. We have evidence that the positive elements do not determine pigment production in vivo but rather modulate transcription of the mouse tyrosinase gene.
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收藏
页码：275 / 278
页数：4
相关论文
共 22 条
[11]   POSITIVE AND NEGATIVE ELEMENTS REGULATE A MELANOCYTE-SPECIFIC PROMOTER [J].
LOWINGS, P ;
YAVUZER, U ;
GODING, CR .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (08) :3653-3662
[12]  
Overbeek Paul A., 1991, Transgenic Research, V1, P31, DOI 10.1007/BF02512994
[13]   A CIS-ACTING ELEMENT INVOLVED IN MOUSE TYROSINASE GENE-EXPRESSION AND PARTIAL-PURIFICATION OF ITS BINDING-PROTEIN [J].
PONNAZHAGAN, S ;
KWON, BS .
PIGMENT CELL RESEARCH, 1992, 5 (04) :155-161
[14]   SUPPRESSION OF TYROSINASE GENE-EXPRESSION BY BROMODEOXYURIDINE IN SYRIAN-HAMSTER MELANOMA-CELLS IS NOT DUE TO ITS INCORPORATION INTO UPSTREAM OR CODING SEQUENCES OF THE TYROSINASE GENE [J].
RAUTH, S ;
DAVIDSON, RL .
SOMATIC CELL AND MOLECULAR GENETICS, 1993, 19 (03) :285-293
[15]   A YEAST ARTIFICIAL CHROMOSOME COVERING THE TYROSINASE GENE CONFERS COPY NUMBER-DEPENDENT EXPRESSION IN TRANSGENIC MICE [J].
SCHEDL, A ;
MONTOLIU, L ;
KELSEY, G ;
SCHUTZ, G .
NATURE, 1993, 362 (6417) :258-261
[16]  
SHIBAHARA S, 1991, J BIOL CHEM, V266, P15895
[17]  
SHIBATA K, 1992, J BIOL CHEM, V267, P20584
[18]  
Silvers WK, 1979, COAT COLORS MICE
[19]  
STEEL KP, 1992, DEVELOPMENT, V115, P1111
[20]  
TAMURA A, 1987, IN VITRO CELL DEV B, V23, P519
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