Typhoid fever and malaria are life threatening illnesses, which are endemic in North Karnataka and causes significant morbidity, mortality and economic loss and are common in most parts of the India and the world. Typhoid fever is caused by bacteria Salmonella typhi and malaria by Plasmodium parasites, which spread to people through bites of infected anopheles mosquitos. These both have become a major public health problem in several parts of the country. About 95% of the population in the country resides in malaria endemic areas. There are chances that the person may get mixed infections, which are usually missed in routine diagnosis by physician in the investigation of pyrexia in the endemic area. Hence, require careful and accurate laboratory diagnosis, which plays an important role to rule out mixed infection and for effective treatment of patients. Here, we present one such case of malaria and typhoid co-infection from a tertiary care hospital of North Karnataka.