VISUALIZATION OF A-BETA-42(43) AND A-BETA-40 IN SENILE PLAQUES WITH END-SPECIFIC A-BETA MONOCLONALS - EVIDENCE THAT AN INITIALLY DEPOSITED SPECIES IS A-BETA-42(43)

被引：1572
作者：
IWATSUBO, T
ODAKA, A
SUZUKI, N
MIZUSAWA, H
NUKINA, N
IHARA, Y
机构：
[1] UNIV TOKYO,FAC MED,INST BRAIN RES,DEPT NEUROL,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,FAC MED,INST BRAIN RES,DEPT NEUROPATHOL,BUNKYO KU,TOKYO 113,JAPAN
[3] TAKEDA CHEM IND,DIV DISCOVERY RES,TSUKUBA,IBARAKI 30042,JAPAN
[4] UNIV TSUKUBA,DEPT NEUROL,TSUKUBA,IBARAKI 305,JAPAN
来源：
NEURON | 1994年 / 13卷 / 01期
关键词：
D O I：
10.1016/0896-6273(94)90458-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
To learn about the carboxy-terminal extent of amyloid beta-protein (A beta) composition of senile plaques (SPs) in the brain affected with Alzheimer's disease (AD), we employed two end-specific monoclonal antibodies as immunocytochemical probes: one is specific for A beta 40, the carboxyl terminus of A beta 1-40, while the other is specific for A beta 42(43). In the AD cortex, all SPs that were labeled with an authentic antibody were A beta 42(43) positive, while only one-third of which, on the average, were A beta 40 positive. There was a strong correlation between A beta 40 positivity and mature plaques. Two familial AD cortices with the mutation of beta-amyloid protein precursor 717 (beta APP717) (Val to Ile) showed a remarkable predominance of A beta 42(43)-positive, A beta 40-negative plaques. Diffuse plaques, representing the earliest stage of A beta deposition, were exclusively positive for A beta 42(43), but completely negative for A beta 40.
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页码：45 / 53
页数：9
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