FLOW-INJECTION STOPPED-FLOW SPECTROFLUOROMETRIC KINETIC DETERMINATION OF TOTAL ASCORBIC-ACID BASED ON AN ENZYME-LINKED COUPLED REACTION

被引：40

作者：

HUANG, HP ^{[1
]}

CAI, RX ^{[1
]}

DU, YM ^{[1
]}

ZENG, YN ^{[1
]}

机构：

[1] WUHAN UNIV,DEPT ENVIRONM SCI,WUHAN 430072,PEOPLES R CHINA

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 309卷 / 1-3期

基金：

中国国家自然科学基金;

关键词：

FLOW INJECTION; KINETIC METHODS; FLUOROMETRY; ENZYMATIC METHODS; LACCASE; ASCORBIC ACID;

D O I：

10.1016/0003-2670(95)00056-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection enzymatic kinetic spectrofluorimetric procedure for determining total ascorbic acid in solution by combining the merging zones principle with the stopped-flow technique is described. It is based on the oxidation of ascorbic acid catalysed by laccase to dehydroascorbic acid, which then reacts with o-phenylenediamine to form a fluorescent quinoxaline. Total ascorbic acid is determined in the range of 0.025-1.0 mu g ml(-1) at 30 determinations per h. The procedure is applied to the determination of total ascorbic acid in wine, beer, urine and pharmaceutical preparations.

引用

页码：271 / 275

页数：5

共 17 条

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