MUTM, A PROTEIN THAT PREVENTS G.C-]T.A TRANSVERSIONS, IS FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE

被引：218
作者：
MICHAELS, ML
PHAM, L
CRUZ, C
MILLER, JH
机构：
[1] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, 405 HILGARD AVE, LOS ANGELES, CA 90024 USA
[2] UNIV CALIF LOS ANGELES, DEPT MICROBIOL, LOS ANGELES, CA 90024 USA
来源：
NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 13期
关键词：
D O I：
10.1093/nar/19.13.3629
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase). An overproducing clone of Fapy-DNA glycosylase complements the original mutM strain that had been isolated after EMS mutagenesis. Thus, we conclude that MutM is actually Fapy-DNA glycosylase. mutM has previously been characterized as a mutator strain that leads specifically to G.C --> T.A transversions. This in vivo characterization correlates well with the mutagenic potential of one of the lesions Fapy-DNA glycosylase removes, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-OxodG).
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页码：3629 / 3632
页数：4
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