PHOSPHOLIPASE-C ISOZYMES IN NEURONS AND GLIAL-CELLS IN CULTURE - AN IMMUNOCYTOCHEMICAL AND IMMUNOCHEMICAL STUDY

被引：32
|
作者：
MIZUGUCHI, M [1 ]
YAMADA, M [1 ]
KIM, SU [1 ]
RHEE, SG [1 ]
机构：
[1] NHLBI,BIOCHEM LAB,BETHESDA,MD 20892
来源：
BRAIN RESEARCH | 1991年 / 548卷 / 1-2期
关键词：
PHOSPHOINOSITIDE 2ND-MESSENGER SYSTEM; TISSUE CULTURE; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING; NEURON; GLIA;
D O I：
10.1016/0006-8993(91)91103-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-sigma) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons > oligodendrocytes > astrocytes for PLC-beta and PLC-gamma, and astrocytes > oligodendrocytes > neurons for PLC-sigma.
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页码：35 / 40
页数：6
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