MULTIPLE-DROP-ARRAY (MDA) TECHNIQUE FOR THE LARGE-SCALE TESTING OF CULTURE MEDIA VARIATIONS IN HANGING MICRODROP CULTURES OF SINGLE CELL SYSTEMS .2. DETERMINATION OF PHYTOHORMONE COMBINATIONS FOR OPTIMAL DIVISION RESPONSE IN NICOTIANA-TABACUM PROTOPLAST CULTURES

被引：13
作者：
HARMS, CT
LORZ, H
POTRYKUS, I
机构：
[1] Friedrich Miescher Institute, CH-4002 Basel
来源：
PLANT SCIENCE LETTERS | 1979年 / 14卷 / 03期
关键词：
D O I：
10.1016/0304-4211(79)90076-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effects on cell division of 7 auxin-like growth substances and 4 cytokinins were examined in Nicotiana tabacum protoplast cultures by the use of the multiple-drop-array (MDA) technique. The phytohormones were tested in 1 auxin/1 cytokinin combinations over a wide range of concentrations. Conditions were found which resulted in routine division frequencies of 90-95% for mesophyll protoplasts of wild-type and two light-sensitive mutants of tobacco. © 1979.
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页码：237 / 244
页数：8
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