INHIBITION OF ENDOPROTEOLYTIC CLEAVAGE OF CYTOMEGALOVIRUS (HCMV) GLYCOPROTEIN-B BY PALMITOYL PEPTIDYL CHLOROMETHYL KETONE

被引：15
作者：
BRUCHER, KH
GARTEN, W
KLENK, HD
SHAW, E
RADSAK, K
机构：
[1] UNIV MARBURG, INST VIROL, ROBERT KOCH STR 17, W-3550 MARBURG, GERMANY
[2] FRIEDRICH MIESCHER INST, CH-4002 BASEL, SWITZERLAND
来源：
VIROLOGY | 1990年 / 178卷 / 02期
关键词：
D O I：
10.1016/0042-6822(90)90365-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Endoproteolytic cleavage of glycoprotein B (gB) of human cytomegalovirus (HCMV) is inhibited by palmitoylated peptidyl-chloromethyl ketone (palFAKR-CEK) at concentrations above 30 μM. Inhibitor treatment of HCMV-infected human fibroblasts neither interfered with exposure of gB on the plasma membrane, detected by surface membrane immunostaining, nor reduced production of intracellular infectious viral progeny. Release of infectious virus, on the other hand, was impaired. © 1990.
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收藏
页码：617 / 620
页数：4
相关论文
共 18 条
[1]   FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].
BONNER, WM ;
LASKEY, RA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88
[2]   SYNTHESIS AND PROCESSING OF THE ENVELOPE GP55-116 COMPLEX OF HUMAN CYTOMEGALOVIRUS [J].
BRITT, WJ ;
AUGER, D .
JOURNAL OF VIROLOGY, 1986, 58 (01) :185-191
[3]   NEUTRALIZING ANTIBODIES DETECT A DISULFIDE-LINKED GLYCOPROTEIN COMPLEX WITHIN THE ENVELOPE OF HUMAN CYTOMEGALOVIRUS [J].
BRITT, WJ .
VIROLOGY, 1984, 135 (02) :369-378
[4]   GROWTH CHARACTERISTICS OF CYTOMEGALOVIRUS IN HUMAN FIBROBLASTS WITH DEMONSTRATION OF PROTEIN-SYNTHESIS EARLY IN VIRAL REPLICATION [J].
FURUKAWA, T ;
FIORETTI, A ;
PLOTKIN, S .
JOURNAL OF VIROLOGY, 1973, 11 (06) :991-997
[5]   INHIBITION OF PROTEOLYTIC ACTIVATION OF INFLUENZA-VIRUS HEMAGGLUTININ BY SPECIFIC PEPTIDYL CHLOROALKYL KETONES [J].
GARTEN, W ;
STIENEKE, A ;
SHAW, E ;
WIKSTROM, P ;
KLENK, HD .
VIROLOGY, 1989, 172 (01) :25-31
[6]   INHIBITION BY MONENSIN OF HUMAN CYTOMEGALOVIRUS DNA-REPLICATION [J].
KAISER, CJ ;
RADSAK, K .
ARCHIVES OF VIROLOGY, 1987, 94 (3-4) :229-245
[7]   CHARACTERIZATION OF MONOCLONAL-ANTIBODIES REACTIVE TO SEVERAL BIOCHEMICALLY DISTINCT HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN COMPLEXES [J].
KARI, B ;
LUSSENHOP, N ;
GOERTZ, R ;
WABUKEBUNOTI, M ;
RADEKE, R ;
GEHRZ, R .
JOURNAL OF VIROLOGY, 1986, 60 (02) :345-352
[8]  
Klenk H D, 1988, Adv Virus Res, V34, P247, DOI 10.1016/S0065-3527(08)60520-5
[9]  
KLENK HD, CURR TOP MICROBIOL I, V80, P19
[10]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
12