LARGE-SCALE ISOLATION OF PIG MUSCLE PHOSPHOGLUCOSE ISOMERASE

被引：7

作者：

GEE, DM

PALMIERI, RH

PORTER, GG

NOLTMANN, EA

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

[2] UNIV CALIF RIVERSIDE,DIV BIOMED SCI,RIVERSIDE,CA 92521

来源：

PREPARATIVE BIOCHEMISTRY | 1979年 / 9卷 / 04期

关键词：

D O I：

10.1080/00327487908061705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A large-scale purification procedure for phosphoglucose isomerase from pig skeletal muscle is described. It consists of two fractionations by selective precipitation and two ion exchange chromatography steps yielding an end product of approximately 900 units (micromoles of substrate converted to product per min per mg of protein, at 30°) specific activity. The method separates three isoenzymic forms with an overall recovery of about 30% of the original total enzyme activity in the form of Isoenzyme III, the latter being the predominant enzyme species. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：441 / 455

页数：15