CONSTRUCTION OF A NEW HOST-VECTOR SYSTEM IN ARTHROBACTER SP AND CLONING OF THE LIPASE GENE

被引：0
|
作者：
MORIKAWA, M [1 ]
DAIDO, H [1 ]
PONGPOBPIBOOL, S [1 ]
IMANAKA, T [1 ]
机构：
[1] OSAKA UNIV,FAC ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1994年 / 42卷 / 2-3期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A square wave pulse of 1 kV/cm electric field strength for 0.5 ms duration yielded 3 x 10(5) transformants/mu g plasmid DNA. The number of transformants increased with the amount of DNA over the range 0.01-5 mu g. This host-vector system was then used successfully to clone and express a lipase gene from Arthrobacter sp. strain MIS38 into both Arthrobacter sp. MIS38 and E. coli JM109.
引用
收藏
页码：300 / 303
页数：4
相关论文
共 50 条
  • [21] Construction of an Arxula adeninivorans host-vector system based on trp1 complementation
    Steinborn, Gerhard
    Wartmann, Thomas
    Gellissen, Gerd
    Kunze, Gotthard
    JOURNAL OF BIOTECHNOLOGY, 2007, 127 (03) : 392 - 401
  • [22] PRELIMINARY DATA ON A HOST-VECTOR SYSTEM FOR CLONING, RESCUING AND EXPRESSING GENES IN HUMAN-CELLS
    BOAST, S
    LANIA, L
    BLASI, F
    ATTI ASSOCIAZIONE GENETICA ITALIANA, 1982, 28 : 56 - 56
  • [23] CONSTRUCTION OF VARIOUS HOST-VECTOR SYSTEMS CARRIED FORWARD TO PLANTS
    SAKAGUCHI, K
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1983, 413 (DEC) : 97 - 98
  • [24] Yeast Kluyveromyces lactis as host for expression of the bacterial lipase: cloning and adaptation of the new lipase gene from Serratia sp.
    Siekstele, Rimantas
    Veteikyte, Ausra
    Tvaska, Bronius
    Matijosyte, Inga
    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 42 (10) : 1309 - 1317
  • [25] Plasmids ofSelenomonas ruminantium and development of host-vector system
    A. Hermanová
    P. Pristaš
    V. Molnárová
    K. Fliegerová
    P. Javorský
    Folia Microbiologica, 2001, 46 : 289 - 291
  • [26] ESTABLISHMENT OF THE HOST-VECTOR SYSTEM FOR MICROMONOSPORA-GRISEORUBIDA
    TAKADA, Y
    INOUYE, M
    MOROHOSHI, T
    MUTO, N
    JOURNAL OF ANTIBIOTICS, 1994, 47 (10) : 1167 - 1170
  • [27] DEVELOPMENT OF A HOST-VECTOR SYSTEM FOR GLUCONOBACTER-SUBOXYDANS
    FUKAYA, M
    OKUMURA, H
    MASAI, H
    UOZUMI, T
    BEPPU, T
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (08): : 2407 - 2411
  • [28] Plasmids of Selenomonas ruminantium and development of host-vector system
    Hermanová, A
    Pristas, P
    Molnárová, V
    Fliegerová, K
    Javorsky, P
    FOLIA MICROBIOLOGICA, 2001, 46 (04) : 289 - 291
  • [29] CONSTRUCTION OF AN EFFICIENT SECRETION HOST-VECTOR SYSTEM AND PRODUCTION OF A HETEROLOGOUS PROTEIN IN BACILLUS-SUBTILIS
    HONJO, M
    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 1993, 67 (05): : 861 - 865
  • [30] STABILITY OF A HOST-VECTOR SYSTEM BASED ON COMPLEMENTATION OF AN ESSENTIAL GENE IN ESCHERICHIA-COLI
    DEGRYSE, E
    JOURNAL OF BIOTECHNOLOGY, 1991, 18 (1-2) : 29 - 40
12345