CONSTRUCTION OF A NEW HOST-VECTOR SYSTEM IN ARTHROBACTER SP AND CLONING OF THE LIPASE GENE

被引：0
|
作者：
MORIKAWA, M [1 ]
DAIDO, H [1 ]
PONGPOBPIBOOL, S [1 ]
IMANAKA, T [1 ]
机构：
[1] OSAKA UNIV,FAC ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1994年 / 42卷 / 2-3期
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D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A square wave pulse of 1 kV/cm electric field strength for 0.5 ms duration yielded 3 x 10(5) transformants/mu g plasmid DNA. The number of transformants increased with the amount of DNA over the range 0.01-5 mu g. This host-vector system was then used successfully to clone and express a lipase gene from Arthrobacter sp. strain MIS38 into both Arthrobacter sp. MIS38 and E. coli JM109.
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页码：300 / 303
页数：4
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