ION TRAP DETECTOR - CAPILLARY GAS-CHROMATOGRAPHY OF VALPROIC ACID AND ITS MONOUNSATURATED METABOLITES IN SERUM USING METHYL-ESTER DERIVATIVES

被引：18
作者：
GAETANI, E [1 ]
LAURERI, CF [1 ]
VITTO, M [1 ]
机构：
[1] UNIV PARMA,IST CHIM FARMACEUT & TOSSICOL,VIA M AZEGLIO 85,I-43100 PARMA,ITALY
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1992年 / 10卷 / 2-3期
关键词：
VALPROIC ACID; GC-MS; METHYL ESTER DERIVATIVES; MONOUNSATURATED METABOLITES;
D O I：
10.1016/0731-7085(92)80028-L
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A quantitative method was developed for valproic acid and five of its mono-unsaturated metabolites using capillary gas chromatography-mass spectrometry with selected ion monitoring. The method was applied to serum and all metabolites were measured in a single run. Methyl esters were synthesized as the derivatives suitable for gas chromatography. Calibration curves were found to be linear and the sensitivities in the order of 0.1-mu-g ml-1. Patients' data are presented. By this method it is possible to separate the stereoisomers of 2-n-propyl-2-pentenoic acid and of 2-n-propyl-3-pentenoic acid.
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页码：193 / 197
页数：5
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