EPIDERMAL GROWTH-FACTOR (EGF) DECREASED ENDOTHELIN-2 (ET-2) PRODUCTION IN HUMAN RENAL ADENOCARCINOMA CELLS

被引：9

作者：

TOKITO, F ^{[1
]}

SUZUKI, N ^{[1
]}

HOSOYA, M ^{[1
]}

MATSUMOTO, H ^{[1
]}

OHKUBO, S ^{[1
]}

FUJINO, M ^{[1
]}

机构：

[1] TAKEDA CHEM IND LTD,TSUKUBA RES LABS,WADAI 10,TSUKUBA,IBARAKI 30042,JAPAN

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

ENDOTHELIN-2; EPIDERMAL GROWTH FACTOR; ENZYME IMMUNOASSAY; ACHN CELL;

D O I：

10.1016/0014-5793(91)81374-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of immunoreactive (ir-) endothelin-2 (ET-2) in renal adenocarcinoma cells, ACHN, was reduced by transforming growth factor-beta, basic fibroblast growth factor, transforming growth factor-alpha and, most strikingly, by epidermal growth factor (EGF). These growth factors did not show such inhibitory effects on the secretion of ir-ET-1 in ET-1-producing cells, indicating that the production of ET-2 and ET-1 is regulated differently by the growth factors. EGF specifically reduced the secretion of not only ir-ET-2 but also ir-big ET-2 with only a small decrease in total protein synthesis. Northern blot analysis indicated that EGF controls the ET-2-production at the transcription levels of ET-2 gene.

引用

页码：17 / 21

页数：5

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