COMPARISON OF THE PROPERTIES OF PURIFIED MITOCHONDRIAL AND CYTOSOLIC RAT-KIDNEY TRANSAMIDINASE

被引：8

作者：

TORMANEN, CD

机构：

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1990年 / 22卷 / 11期

关键词：

D O I：

10.1016/0020-711X(90)90305-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Mitochondrial rat kidney transamidinase was solubilized by two extractions with the surfactant Zwittergent 3-14. 2. 2. Mitochondrial and cytosolic forms of rat kidney transamidinase were purified by chromatography on DEAE-Trisacryl M, phenyl-Sepharose C1-4B and hydroxylapatite columns. 3. 3. The specific activity of purified mitochondrial enzyme was significantly higher than purified cytosolic enzyme. 4. 4. The subunit molecular mass, the electrophoretic mobility under nondenaturing conditions, and the activation energy were similar for purified mitochondrial and cytosolic transamidinase. © 1990.

引用

页码：1243 / 1250

页数：8

共 15 条

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[2]

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