SOME PROPERTIES OF DIAZEPAM-H-3 DISPLACING ACTIVITY FROM HUMAN-URINE

被引：74
作者：
NIELSEN, M
GREDAL, O
BRAESTRUP, C
机构：
[1] Psychopharmacological Research Laboratory, Sct. Hans Mental Hospital, Dept. E
[2] A/S Ferrosan, DK-2860 Soeborg
来源：
LIFE SCIENCES | 1979年 / 25卷 / 08期
关键词：
D O I：
10.1016/0024-3205(79)90509-5
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
A potent 3H-diazepam displacing factor (IC50 < 0.2 μg/ml) is isolated from human urine by adsorption to Chromosorb, elution with 50% ethanol, heating at pH 1, extractions into ethyl acetate and diethyl ether, and separation on a Sephadex LH20 column. The lipophilic compound has a molecular weight below 500 daltons and is inactivated by chymotrypsin or by exposure to pH > 12. The 3H-diazepam displacing material is probably present in urine as a conjugate or in a proform. © 1979.
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页码：679 / 686
页数：8
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