CHARACTERIZATION OF THE GENES-CODING FOR THE F1F0 SUBUNITS OF THE SODIUM-DEPENDENT ATPASE OF PROPIONIGENIUM-MODESTUM

被引：10

作者：

KRUMHOLZ, LR ^{[1
]}

ESSER, U ^{[1
]}

SIMONI, RD ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT BIOL SCI,STANFORD,CA 94305

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 91卷 / 01期

关键词：

SODIUM; ATPASE; PROPIONIGENIUM-MODESTUM; DNA SEQUENCE;

D O I：

10.1111/j.1574-6968.1992.tb05180.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DNA coding for the eight structural genes and uncI of the sodium dependent ATPase of Propionigenium modestum has been cloned and sequenced. Based on sequence homology, the genes were determined to appear in the order uncBEFHAGDC as in several other bacterial species. Minicell experiments revealed that plasmids containing the P. modestum DNA expressed those ATPase polypeptides in Escherichia coli. These were very similar in molecular mass to those obtained from the purified ATPase of P. modestum. No membrane-bound ATPase activity was observed in E. coli unc deletion strains containing the P. modestum ATPase genes. Amino acid alignments which were done with the Fo subunits revealed only a few conservative changes in the highly conserved regions of the polypeptides.

引用

页码：37 / 41

页数：5

共 12 条

[1] CLONING AND SEQUENCING OF THE GENE ENCODING THE BETA SUBUNIT OF THE SODIUM-ION TRANSLOCATING ATP SYNTHASE OF PROPIONIGENIUM-MODESTUM [J].