NUCLEOTIDE-SEQUENCE OF THE ZUCCHINI YELLOW MOSAIC-VIRUS CAPSID-ENCODING GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：33

作者：

GALON, A ^{[1
]}

ANTIGNUS, Y ^{[1
]}

ROSNER, A ^{[1
]}

RACCAH, B ^{[1
]}

机构：

[1] AGR RES ORG,VOLCANI CTR,DEPT VIROL,POB 6,IL-50250 BET DAGAN,ISRAEL

来源：

GENE | 1990年 / 87卷 / 02期

关键词：

coat protein; cucurbits; plants; potyviruses; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90312-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Zucchini yellow mosaic virus (ZYMV) RNA was purified and used as a template for the synthesis of cDNA. A partial restriction map covering 9.4 kb of the ZYMV genome was constructed from three clones designated ZYKS-22, ZYKS-16 and ZYKS-3. Sequencing the 3′-end region of the ZYMV genome indicates the presence of (A)48 chain. This is followed by an untranslated region of 210 nucleotides (nt) and a coding region of 837 nt corresponding to the putative virus coat protein (Cp) gene (cp). The predicted amino acid (aa) sequence of Cp derived from the cDNA showed about 50% to 62% homology with the known aa sequence for Cp of six other potyviruses. A construct of the putative cp was subcloned in frame with the lacZp gene promoter in a Bluescript plasmid and expressed in Escherichia coli cells. The fusion polypeptides (34 and 41 kDa), positively reacted in Western blots with an antiserum prepared against the native virus Cp. © 1990.

引用

页码：273 / 277

页数：5

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